Dr. Mishra has made some major innovative contributions to analytical fluorimetry, especially in the area of analyzing complex multifluorophoric systems. Some of them include:
Introduction of a novel concept for analyzing multifluorophoric systems using synchronous fluorescence spectroscopy (SFS) by a modification of Beer-Lambert’s Law. This research provides a conceptual foundation for understanding ‘concentration dependent red shift’ observed in Synchronous Fluorescence (SF) Spectroscopy based analysis of complex multifluorophoric systems and also introduces a protocol for SF analysis.
Introduction of ‘Total Synchronous Fluorescence Spectroscopy’ (TSFS) as an attractive alternative to the existing Excitation-Emission Matrix Spectroscopy, and a procedure for theapplication of PARAFAC (a chemometric technique) to TSFS data enabling automation of data processing.
Introduction of a facile measurement technique for luminescence quantum yield , by designing an optical spectrometer that uses the same light source and detector for both the electronic absorption and luminescence spectral measurements.
Introduction of a new fluorescence data collection concept, ‘White Light Excitation Fluorescence’ and showcasing its application potential.
Introduction of miniaturized fiber-optic compatible fluorimeter configurations and probe-heads.
Introduction of a new parameter called ‘total fluorescence quantum yield’ relating to the volume under the 3D EEMF plots and the method of ‘excitation emission matrix spectral subtraction fluorescence’ .
Innovative optimization of fluorescence data acquisition techniques and various chemometric methods towards developing practical applications like petroleum fuel adulteration analysis , petroleum fuel-biofuel blend composition estimation , monitoring of transformer oil degradation , analysis of polycyclic aromatic compounds in water, classification of Ayurvedic preparations and oral cancer diagnosis.